rabbit polyclonal anti-syn1  (Synaptic Systems)

Journal: Cell Reports Medicine

Article Title: Increased intestinal bile acid absorption contributes to age-related cognitive impairment

doi: 10.1016/j.xcrm.2024.101543

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-SYN1 , Proteintech , Cat#20258-1-AP, RRID: AB_2800493.

Techniques: Recombinant, Saline, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Western Blot, Software, Membrane, Microscopy

Journal: Cell reports. Medicine

Article Title: Increased intestinal bile acid absorption contributes to age-related cognitive impairment.

doi: 10.1016/j.xcrm.2024.101543

Figure Lengend Snippet: Figure 2. The excess of CPBA and ammonia in the brain is associated with hippocampal synapse loss in aging rodents (A‒D and I‒L) The percentages of CPBAs and ammonia level as well as the CPBA composition (e.g., taurocholic acid [TCA]) in the brain of rodents in both genders (n = 6–8/group). (E, F, M, and N) The representative images and counts of hippocampal synapses in rats and mice resulted from transmission electron microscopy (n = 6–8/group, 3 repeats/sample). (G and O) The concentrations of hippocampal Synapsin 1 (Syn1) in both genders of rodents (n = 68/group, 3 repeats/sample). (H and P) Pearson’s r correlation between hippo- campal Syn1 level and brain TCA or ammonia in rodents. The comparisons of CPBA, ammonia, and synapse data in rodents were performed with two-way ANOVA. The statistical difference is labeled as follows: ***p < 0.005 vs. 24-week female (F24); ##p < 0.01; ###p < 0.005 vs. 24-week male (M24); $$p < 0.01 vs. 111-week male (M111) for the comparison of the CBPA composition among groups; *p < 0.05; **p < 0.01; ***p < 0.001 for other comparisons. See also Figures S2 and S3.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SYN1 Proteintech Cat#20258-1-AP, RRID: AB_2800493 Mouse monoclonal anti-PSD95 Abcam Cat#20665-1-AP, RRID: AB_300453 Rabbit monoclonal anti-b-Actin Cell Signaling Technology Cat#4970, RRID: AB_2223172 Anti-rabbit, HRP-linked Cell Signaling Technology Cat#7074, RRID: AB_2099233 Anti-mouse, HRP-linked Cell Signaling Technology Cat#7076, RRID: AB_330924 Anti-rabbit, Alex Flour 488-linked Abcam Cat#ab150077, RRID: AB_2630356 Anti-mouse, Alex Flour 647-linked Abcam Cat#ab150179, RRID: AB_2884038 Biological samples Serum from mice This paper N/A Tissues from mice This paper N/A Chemicals, peptides, and recombinant proteins Phosphate buffered saline Sigma Cat#P2272, CAS: NA Paraformaldehyde Sigma Cat#158127, CAS: 30525-89-4 RIPA buffer Beyotime Biotechnology Cat#P0013C, CAS: NA DNase I Sigma Cat#11284932001, CAS: NA Papain Sigma Cat#232-627-2, CAS: 9001-73-4 B-27 Gibco Cat#17504044 L-glutamine Gibco Cat#25030149 Critical commercial assays Asbt Elisa kit (for rat) Jiangsu Mei Biao Biological Technology Co., Ltd. Cat#MB-7410A Asbt Elisa kit (for mouse) Jiangsu Mei Biao Biological Technology Co., Ltd. Cat#MB-6453A Syn1 Elisa kit (for rat) S.A.B Cat#EK3880 Syn1 Elisa kit (for mouse) S.A.B Cat#EK6728-2 Ammonia Assay Kit Sigma-Aldrich Cat#AA0100, SYBR Green PCR Master Mix Vazyme Cat#A25742 Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227 Clarity Western ECL Substrate Bio-Rad Cat#1705060 Experimental models: Cell lines Rat primary hippocampal neurons This paper N/A Experimental models: Organisms/strains Wistar rat Vital River laboratory animal technology Co. Ltd., Beijing, China N/A C57BL/6J mouse Shanghai SLAC Laboratory Animal Co., Ltd N/A Software and algorithms GraphPad Prism 9.3 software GraphPad Software http://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/illustrator.html TargetLynx 4.2 Waters Corporation N/A Microsoft 365 Microsoft https://www.microsoft.com/zh-cn/ microsoft-365/buy/compare-allmicrosoft-365-products?tab=1 (Continued on next page) e1 Cell Reports Medicine 5, 101543, May 21, 2024

Techniques: Transmission Assay, Electron Microscopy, Labeling, Comparison